The black, heavily guarded limousine experienced mechanical problems in Tel Aviv before Mr Obama landed in the country for a state visit.
Media reports said the vehicle was mistakenly filled with diesel rather than petrol.
The disabled limo was swapped for a back-up, and Mr Obama’s busy itinerary in Tel Aviv and Jerusalem on Wednesday was not affected.
“This is why we bring multiple vehicles and a mechanic on all trips,” US Secret Service spokesman Edwin Donovan said in a statement.
“Situations like this are planned for extensively by our advance teams so that the President’s itinerary is unaffected by these types of issues.”